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PPEPTECH and BIOTECH extract DNA from Kiwi fruit
and conduct DNA fingerprinting!!!
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DNA Extraction from Kiwi fruit: Students break down the plant's cell walls by mashing the fruit in a ziplock bag in a solution of soap (to break the cellular and nuclear membranes) and salt (to precipitate proteins). The DNA is released from nucleus into the aqueous part of their mixture. After the students separate the solids (preciptated proteins, cellular debri, seeds, etc) from the liquid, they add ethanol to precipate the DNA. |
DNA fingerprinting: Cat Food Caper--the students helped figure out who has been eating Fluffy's food.
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| Step 1: Set up the electrophoresis chamber place stoppers at each end of the casting tray and comb closest to the black (negative) electrodes. |
Step 2: Pour the hot agarose into the gel tray and let set for 10 minutes to solidify. |
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Step 3: Pipet DNA sample out of microcentrifuge tube. |
Step 4: Pipet the sample into one of the wells that you made in the agarose with the comb. |
This lab and others are available online. Contact the BIOTECH Project to check out the
materials or to have the Biotechnology Mobile Laboratory visit your classroom.
BIOTECH Home | Laboratory Activities | Biotechnology Resources
On Tour with BIOTECH | BIOTECH Bulletin Board
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BIOTECH Project Department of Molecular and Cellular Biology The University of Arizona August 15, 2000
Nadja Anderson, Ph.D.
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