UA Plant Genomics Labs
Summer Schedule of
weekly meeting Summer 2003 Marley Room 341H Thursday 12:00-1:30
Poster Session--- July 22, 2003
Arabidopsis and Plant Genome Resources
Laboratory Activities
Summer 2003 Activities designed by interns to take back to the classroom
BIOTECH
Home
Gateway to the BIOTECH Project
PREP Handbook with activities using Arabidopsis thaliana
The University of Arizona
Department of Molecular and Cellular
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Teacher Internship in Plant Genomics Plant Genomics Labs |
Judith Brown: 1) Construction and characterization of a subtracted whitefly EST library. 2) Construction and characterization of salivary gland/gut library from viruliferous whiteflies. 3) Whitefly endosymbiont library construction and EST sequencing.
Vicki Chandler: 1) Characterize transgenic maize plants carrying dsRNA silencing constructs targeting chromatin protein encoding genes by DNA gel blot analysis of introduced DNA and RT-PCR analysis of target RNA levels. 2) Participate in crossing transgenes into appropriate lines for assaying effects on paramutation at b and on transgene silencing.
David Galbraith: Characterize Arabidopsis mutants carrying mutations in specific cytochrome P450 genes
David Gang: Plants produce an amazing diversity of small chemicals, used by the plant to fight off herbivores and pests and by people as medicinal and flavoring agents. My lab uses modern tools of biochemistry, genomics, and analytical chemistry to investigate how these important compounds are produced by plants. In particular, we are interested in the anti-inflammatory components of ginger and turmeric and in the flavor compounds in sweet basil.
Rich Jorgensen: 1) Investigate alternative splicing of messenger RNAs in Arabidopsis. 2) Use gene silencing to do functional genomics in polyploid plants.
Brian Larkins: 1) Characterize the regulation of maize endosperm development as it relates to protein synthesis and protein quality. 2) Determine the role of DNA endoreduplication in starch and protein synthesis. 3) Functional genomics of kernel development based on analysis of Mutator-induced mutants.
Marc Orbach: Create an insertion mutant library of Magnaporthe grisea, a fungal pathogen of rice, using a promoterless reporter cassette to allow screening for expression of genes that the construct inserts into. Phenotypic analysis of the insertion strains, involving both visual and biochemical analysis of the transformed lines.
Karen Schumaker: 1) Compare the function of salt and osmotic tolerance determinants and the associated regulatory pathways in a salt-tolerant plant (salt cress, a halophyte) and its salt-sensitive relative (Arabidopsis, a glycophyte). 2) Functionally determine the role of SOS2-like protein kinases in the response of Arabidopsis to environmental cues and abiotic stresses 3) Functionally determine the role of chromatin regulatory proteins in the response of Arabidopsis to abiotic stress.
Elizabeth Vierling: 1) Define natural genetic variation in plant heat tolerance. 2) Map genes for heat tolerance using molecular methods. 3) Proteomic analysis of differences between mutant plants.Rod Wing: High-throughput genomics institute focusing on structural and functional genomics of crop plants including sequencing, physical mapping, BAC libraries, EST libraries and functional genomics.
Ramin Yadegari: We use genetic and molecular approaches to understand how seeds develop in response to fertilization in flowering plants. The intern will: 1) Analyze the expression of important regulatory genes during plant development and growth; and 2) Participate in analysis of mutants with defects in seed development.
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Center for Plant Genomics Outreach Program collaboration between Departments of Plant Sciences and Molecular and Cellular Biology The University of Arizona Nadja Anderson, Ph.D. |